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rabbit anti-syntaxin-16  (Synaptic Systems)


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    Synaptic Systems rabbit anti-syntaxin-16
    Summary of SV, SNARE, and other trafficking proteins enriched in VGluT3 immunoisolates at P23 and identified by MS analysis
    Rabbit Anti Syntaxin 16, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-syntaxin-16/product/Synaptic Systems
    Average 90 stars, based on 1 article reviews
    rabbit anti-syntaxin-16 - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells"

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1016/j.mcpro.2023.100704

    Summary of SV, SNARE, and other trafficking proteins enriched in VGluT3 immunoisolates at P23 and identified by MS analysis
    Figure Legend Snippet: Summary of SV, SNARE, and other trafficking proteins enriched in VGluT3 immunoisolates at P23 and identified by MS analysis

    Techniques Used: Membrane

    Immunolocalization analysis of trafficking proteins in the adult organ of Corti . A and B , Syntaxin-6 ( A ) and syntaxin-16 ( B ), enriched in our MS experiments, were previously shown to be expressed in IHCs. C–F , The Golgi and endosomal markers YKT6 ( C ) and VtiA ( D ), the ER and presynaptic plasma membrane protein VAP-A ( E ), the ER protein VCP ( F ) are expressed in IHCs. Note that all proteins localize to the basolateral region of the IHCs, although in some cases the protein is also expressed in afferent (postsynaptic) and efferent fibers of SGNs. Images correspond to high magnification views of representative P15–25 IHCs immunolabeled with antibodies against the candidate proteins ( red ), the ribbon marker CtBP2/RIBEYE ( green ), and the IHC marker otoferlin ( blue ). In ( C–F ), the upper panels show overviews of representative IHCs, displaying maximum intensity projections of 5 to 10 confocal optical sections through the longitudinal axis of the IHCs (scale bars: 5 μm). The bottom panels show a zoom into the synaptic area, displaying single confocal optical sections through the longitudinal axis of a single IHC at the basal region (scale bars: 2 μm). IHC, inner hair cell; SGN, spiral ganglion neuron.
    Figure Legend Snippet: Immunolocalization analysis of trafficking proteins in the adult organ of Corti . A and B , Syntaxin-6 ( A ) and syntaxin-16 ( B ), enriched in our MS experiments, were previously shown to be expressed in IHCs. C–F , The Golgi and endosomal markers YKT6 ( C ) and VtiA ( D ), the ER and presynaptic plasma membrane protein VAP-A ( E ), the ER protein VCP ( F ) are expressed in IHCs. Note that all proteins localize to the basolateral region of the IHCs, although in some cases the protein is also expressed in afferent (postsynaptic) and efferent fibers of SGNs. Images correspond to high magnification views of representative P15–25 IHCs immunolabeled with antibodies against the candidate proteins ( red ), the ribbon marker CtBP2/RIBEYE ( green ), and the IHC marker otoferlin ( blue ). In ( C–F ), the upper panels show overviews of representative IHCs, displaying maximum intensity projections of 5 to 10 confocal optical sections through the longitudinal axis of the IHCs (scale bars: 5 μm). The bottom panels show a zoom into the synaptic area, displaying single confocal optical sections through the longitudinal axis of a single IHC at the basal region (scale bars: 2 μm). IHC, inner hair cell; SGN, spiral ganglion neuron.

    Techniques Used: Membrane, Immunolabeling, Marker



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    Image Search Results


    Summary of SV, SNARE, and other trafficking proteins enriched in VGluT3 immunoisolates at P23 and identified by MS analysis

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells

    doi: 10.1016/j.mcpro.2023.100704

    Figure Lengend Snippet: Summary of SV, SNARE, and other trafficking proteins enriched in VGluT3 immunoisolates at P23 and identified by MS analysis

    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).

    Techniques: Membrane

    Immunolocalization analysis of trafficking proteins in the adult organ of Corti . A and B , Syntaxin-6 ( A ) and syntaxin-16 ( B ), enriched in our MS experiments, were previously shown to be expressed in IHCs. C–F , The Golgi and endosomal markers YKT6 ( C ) and VtiA ( D ), the ER and presynaptic plasma membrane protein VAP-A ( E ), the ER protein VCP ( F ) are expressed in IHCs. Note that all proteins localize to the basolateral region of the IHCs, although in some cases the protein is also expressed in afferent (postsynaptic) and efferent fibers of SGNs. Images correspond to high magnification views of representative P15–25 IHCs immunolabeled with antibodies against the candidate proteins ( red ), the ribbon marker CtBP2/RIBEYE ( green ), and the IHC marker otoferlin ( blue ). In ( C–F ), the upper panels show overviews of representative IHCs, displaying maximum intensity projections of 5 to 10 confocal optical sections through the longitudinal axis of the IHCs (scale bars: 5 μm). The bottom panels show a zoom into the synaptic area, displaying single confocal optical sections through the longitudinal axis of a single IHC at the basal region (scale bars: 2 μm). IHC, inner hair cell; SGN, spiral ganglion neuron.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells

    doi: 10.1016/j.mcpro.2023.100704

    Figure Lengend Snippet: Immunolocalization analysis of trafficking proteins in the adult organ of Corti . A and B , Syntaxin-6 ( A ) and syntaxin-16 ( B ), enriched in our MS experiments, were previously shown to be expressed in IHCs. C–F , The Golgi and endosomal markers YKT6 ( C ) and VtiA ( D ), the ER and presynaptic plasma membrane protein VAP-A ( E ), the ER protein VCP ( F ) are expressed in IHCs. Note that all proteins localize to the basolateral region of the IHCs, although in some cases the protein is also expressed in afferent (postsynaptic) and efferent fibers of SGNs. Images correspond to high magnification views of representative P15–25 IHCs immunolabeled with antibodies against the candidate proteins ( red ), the ribbon marker CtBP2/RIBEYE ( green ), and the IHC marker otoferlin ( blue ). In ( C–F ), the upper panels show overviews of representative IHCs, displaying maximum intensity projections of 5 to 10 confocal optical sections through the longitudinal axis of the IHCs (scale bars: 5 μm). The bottom panels show a zoom into the synaptic area, displaying single confocal optical sections through the longitudinal axis of a single IHC at the basal region (scale bars: 2 μm). IHC, inner hair cell; SGN, spiral ganglion neuron.

    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).

    Techniques: Membrane, Immunolabeling, Marker

    EHD2 KD in 3T3-L1 adipocytes is associated with impaired insulin signaling. (A) Representative Western blots of total phospho-tyrosine in 3T3-L1 adipocyte lysates collected 96 h after gene silencing with control siRNA or EHD2 siRNA, either untreated or following 20-min stimulation with 100 nM insulin ( n = 2) and of protein levels of EHD2, SNARE proteins Syntaxin4 (Sx4), SNAP23, VAMP2, and Syntaxin16 (Sx16), as well as regulatory protein Munc18c, and GLUT4 ( n = 3). GAPDH was used as a loading control and the calculations displayed in C were performed exclusively on basal samples. (B) Glucose uptake (2-deoxy-D-glucose) of 3T3-L1 adipocytes, 96 h after gene silencing with control siRNA (Control) or EHD2 siRNA (EHD2 KD), with (INS) or without (Basal) 20-min stimulation with 100 nM insulin. Data were corrected for nonspecific cellular isotope uptake by performing parallel assays in the presence of 10 μM cytochalasin B and normalized to those obtained in the insulin-stimulated control adipocytes for each data set. Mean ± SD of n = 4 independent experiments are shown. Statistical analysis was done using two-way ANOVA Tukey’s Honest Significant Difference (TukeyHSD), * p < 0.05. (C) Corresponding quantification of protein expression in EHD2 siRNA KD adipocytes in the absence of an acute insulin challenge (lanes labeled “-” in A) is normalized to GAPDH and expressed as a percentage of protein expression in control siRNA adipocytes. Mean and SD of n = 3 independent experiments are shown. Statistical analysis was conducted using unpaired two-sample t test, * p < 0.05, ** p < 0.01.

    Journal: Molecular Biology of the Cell

    Article Title: EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events

    doi: 10.1091/mbc.E23-03-0078

    Figure Lengend Snippet: EHD2 KD in 3T3-L1 adipocytes is associated with impaired insulin signaling. (A) Representative Western blots of total phospho-tyrosine in 3T3-L1 adipocyte lysates collected 96 h after gene silencing with control siRNA or EHD2 siRNA, either untreated or following 20-min stimulation with 100 nM insulin ( n = 2) and of protein levels of EHD2, SNARE proteins Syntaxin4 (Sx4), SNAP23, VAMP2, and Syntaxin16 (Sx16), as well as regulatory protein Munc18c, and GLUT4 ( n = 3). GAPDH was used as a loading control and the calculations displayed in C were performed exclusively on basal samples. (B) Glucose uptake (2-deoxy-D-glucose) of 3T3-L1 adipocytes, 96 h after gene silencing with control siRNA (Control) or EHD2 siRNA (EHD2 KD), with (INS) or without (Basal) 20-min stimulation with 100 nM insulin. Data were corrected for nonspecific cellular isotope uptake by performing parallel assays in the presence of 10 μM cytochalasin B and normalized to those obtained in the insulin-stimulated control adipocytes for each data set. Mean ± SD of n = 4 independent experiments are shown. Statistical analysis was done using two-way ANOVA Tukey’s Honest Significant Difference (TukeyHSD), * p < 0.05. (C) Corresponding quantification of protein expression in EHD2 siRNA KD adipocytes in the absence of an acute insulin challenge (lanes labeled “-” in A) is normalized to GAPDH and expressed as a percentage of protein expression in control siRNA adipocytes. Mean and SD of n = 3 independent experiments are shown. Statistical analysis was conducted using unpaired two-sample t test, * p < 0.05, ** p < 0.01.

    Article Snippet: Mammalian uncoordinated-18c (Munc18c; #116 202, RRID:AB_2619785), Syntaxin 4 (Sx4; #110 042, RRID:AB_887853), vesicle-associated membrane protein 2 (VAMP2; #104 403, RRID:AB_2864782), synaptosome associated protein 23 (SNAP23; #111 213, RRID:AB_10805651), and Syntaxin 16 (Sx16; #110 162, RRID:AB_887799) were all from Synaptic Systems (Coventry, UK).

    Techniques: Western Blot, Expressing, Labeling

    Summary of mass spectrometry data of Sx16 coimmunoprecipitation analysis.

    Journal: Molecular Biology of the Cell

    Article Title: EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events

    doi: 10.1091/mbc.E23-03-0078

    Figure Lengend Snippet: Summary of mass spectrometry data of Sx16 coimmunoprecipitation analysis.

    Article Snippet: Mammalian uncoordinated-18c (Munc18c; #116 202, RRID:AB_2619785), Syntaxin 4 (Sx4; #110 042, RRID:AB_887853), vesicle-associated membrane protein 2 (VAMP2; #104 403, RRID:AB_2864782), synaptosome associated protein 23 (SNAP23; #111 213, RRID:AB_10805651), and Syntaxin 16 (Sx16; #110 162, RRID:AB_887799) were all from Synaptic Systems (Coventry, UK).

    Techniques: Mass Spectrometry